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SRX5823262: WGS of Bifidobacterium pseudolongum, strain NM87_A27A: murine gut isolate
1 ILLUMINA (Illumina NovaSeq 6000) run: 2M spots, 551.6M bases, 163.3Mb downloads

Design: Sequencing performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg Biohub. Nextera XT kits were used to make the libraries with CZ Biohubs custom designed adapters - 12 bp index sequences on each side of the library and are dual unique so that each sequence only appears once. The libraries were made in 384 well plates in 4 uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the library was pooled for sequencing. Sequencing was performed on a NovaSeq S4 flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps with dual 12 bp index sequences.
Submitted by: University of Toronto
Study: Genome sequencing and assembly - library of cultured bacterial species from laboratory mouse gut microbiota from the University of Toronto
PRJNA474907 • SRP193474 • All experiments • All runs
show Abstracthide Abstract
Bank of bacterial mono-isolates from laboratory mouse gut microbiota
Sample:
SAMN11366404 • SRS4751447 • All experiments • All runs
Organism: Bifidobacterium pseudolongum
Library:
Name: NM87_A27A
Instrument: Illumina NovaSeq 6000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 2M spots, 551.6M bases, 163.3Mb
Run# of Spots# of BasesSizePublished
SRR90467371,969,853551.6M163.3Mb2019-05-12

ID:
7828980

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